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KMID : 1234420080360020165
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Korean Journal of Microbiololgy and Biotechnology
2008 Volume.36 No. 2 p.165 ~ p.172
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Effect of Temperature and pH on the Biosorption of Heavy Metals by Exophiala sp
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Lim Joung-Su
Lee So-Jin
Lee Eun-Young
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Abstract
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Hwaseong, Korea To find the optimum growth and metal removal condition of isolated strain LH2, effects of the environmental factors such as medium pH, growth temperature, and metal concentrations were investigated. Based on the 18S rDNA analysis, the isolated strain was identified to Exophiala sp. with 100% homology. Isolated strain Exophiala sp. LH2 showed maximum removal efficiency of metals at the shaking conditions of pH 7 and 25oC. When the concentration of metal was under 200 ppm, the specific metal removal velocity at pH 7 increased from 0.01 to 4.43 mg-metal ·L-1 · d-1·mg ·DCW-1 as the concentration of metal increased from 10 ppm to 200 ppm. When 200 ppm of each metal was contained in the culture medium adjusted with pH 7, metal removal efficiencies of Cr, Cu, Ni, Pb and Zn were 99.28%, 97.67%, 91.94%, 99.77%, 99.61%, respectively.
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KEYWORD
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Exophiala sp., biosorption, heavy metals
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